Tachykinin 3 FLISA Kit
Quick Overview for Tachykinin 3 FLISA Kit (ABIN2871166)
Target
See all Tachykinin 3 (TAC3) FLISA KitsReactivity
Detection Method
Method Type
Application
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Analytical Method
- Quantitative
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Tachykinin 3 (TAC3) ELISA Kit
TAC3 Reactivity: Human Colorimetric Competition ELISA 9.88 pg/mL - 800 pg/mL Plasma, Serum
Tachykinin 3 (TAC3) ELISA KitTAC3 Reactivity: Rat Colorimetric Competition ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tachykinin 3 (TAC3) ELISA KitTAC3 Reactivity: Human Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Tachykinin 3 (TAC3) ELISA KitTAC3 Reactivity: Human Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Tachykinin 3 (TAC3)
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Alternative Name
- Neurokinin B
Target See all Tachykinin 3 (TAC3) FLISA Kits
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