APL 1beta-28 FLISA Kit
The Human APL 1beta-28 ELISA Kit (ABIN2871323) is a Fluorometric ELISA Kit designed to quantify Human APL 1beta-28.
Catalog No. ABIN2871323
Shipping to:
United States
Contact our Customer Service for availability and price in your country.
Contact Info
Quick Overview for APL 1beta-28 FLISA Kit (ABIN2871323)
Target
APL 1beta-28
Reactivity
Human
Detection Method
Fluorometric
Method Type
Competition ELISA
Detection Range
51-1125 pg/mL
Application
ELISA
-
-
Minimum Detection Limit
- 51 pg/mL
-
Analytical Method
- Quantitative
-
Sensitivity
- 51 pg/mL
-
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Pre-coated
-
Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
-
- APL 1beta-28
Target
-
You are here:
