Topoisomerase I ELISA Kit

Catalog No. ABIN2947065

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Topoisomerase I.

Quick Overview for Topoisomerase I ELISA Kit (ABIN2947065)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details Topoisomerase I ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Mouse Topoisomerase I (TOP1) ELISA Kit

Analytical Method: Quantitative

Sensitivity: 46.9 pg/mL

Components: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Material not included:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Application Details

Application Notes: Optimal dilutions/concentrations should be determined by the end user.

Comment:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plate: Pre-coated

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Topoisomerase I (TOP1) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Topoisomerase I (TOP1) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Expiry Date: 6 months

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: Topoisomerase I

Background:

Ensembl: ENSMUST00000109468

UniProt Entry Name: TOP1_MOUSE

Gene ID: 21969

NCBI Accession: NP_033434

UniProt: Q04750

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance