MAPK14 ELISA Kit

Catalog No. ABIN2950156

This Mouse MAPK14 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse MAPK14.

Quick Overview for MAPK14 ELISA Kit (ABIN2950156)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.25 ng/mL - 80 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details MAPK14 ELISA Kit

Minimum Detection Limit: 1.25 ng/mL

Purpose: Mouse Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA Kit

Analytical Method: Quantitative

Sensitivity: 0.75 ng/mL

Components: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Material not included:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Application Details

Application Notes: Optimal dilutions/concentrations should be determined by the end user.

Comment:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plate: Pre-coated

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of MAPK14 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of MAPK14 were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Expiry Date: 6 months

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: Mitogen-Activated Protein Kinase 14

Background:

Ensembl: ENSMUST00000004990 [P47811-3], ENSMUST00000062694 [P47811-1], ENSMUST00000114752 [P47811-4], ENSMUST00000114754 [P47811-4], ENSMUST00000114758 [P47811-2]

UniProt Entry Name: MK14_MOUSE

Gene ID: 26416

NCBI Accession: NP_036081

UniProt: P47811

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins