Quick Overview for Nitrite Determination Assay Kit (ABIN7384583)
Detection Method
Application
Sample Type
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Detection Range
- 1-100 μM
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Minimum Detection Limit
- 1 μM
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Purpose
- The kit is designed for nitrite determination in biological samples.
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Specificity
- Specific for nitrite determination in plasma samples.
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Characteristics
- The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
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Components
- Plate and the reagents neccesary to perform the assay.
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Material not included
- Pipettes, reaction tubes, plate reader.
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High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
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Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 Reactivity: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 Reactivity: Human Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
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Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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Sample Volume
- 50 μL
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Assay Time
- 1 h
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Plate
- Uncoated
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Reagent Preparation
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No reagent preparation is needed.
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Sample Preparation
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Plasma samples may be deproteinized before performing the assay.
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Assay Procedure
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Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 ( see in booklet) . Use ultrapure water as diluent. Performing the assay: 1. Add 50 μL of samples or standards in each well (96-well plate). 2.Add 50 μL of Reagent A in each well. Incubate for 10 minutes protected from light. 3.Add 50 μL of Reagent B in each well. Incubate for 10 minutes protected from light. 4.Read the absorbance at 540 nm within 30 minutes.
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Calculation of Results
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Determine average absorbance value of each experimental sample. Determine its concentration by comparison to the Nitrite Standard reference curve (Figure 2 see images). Nitrite (μM) = (?A540nm- intercept)/ slope
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Restrictions
- For Research Use only
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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