IL-4 ELISA Kit

Catalog No. ABIN411300

This Mouse IL-4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-4. This ELISA Kit has been cited in 31+ publications.

Quick Overview for IL-4 ELISA Kit (ABIN411300)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Binding Specificity: AA 21-140

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details IL-4 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-4

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: H21-S140

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: H21-S140

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli, H21-S140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-4 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse IL-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-4 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 44, Standard deviation: 2.38, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 168, Standard deviation: 7.9, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 283, Standard deviation: 17.55, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 57, Standard deviation: 3.88, CV(%): 6.8
• Sample 2: n=24, Mean(pg/ml): 185, Standard deviation: 10.36, CV(%): 5.6
• Sample 3: n=24, Mean(pg/ml): 326, Standard deviation: 23.8, CV(%): 7.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-4 ELISA Kit (ABIN411300)

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." in: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, (2018) (PubMed).

Han, Zhang, Tan, Liu, Ma, Ou-Yang, Wu: "EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 1008-1016, (2018) (PubMed).

Yang, Zhang, Cai, Shang: "Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 818-828, (2018) (PubMed).

Hu, Feng, Wang, Song, Ni: "5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production." in: Iranian journal of basic medical sciences, Vol. 21, Issue 4, pp. 364-369, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." in: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Wang, Zhang, Wang, Gadahi, Xu, Yan, Song, Li: "Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis." in: Frontiers in microbiology, Vol. 8, pp. 168, (2017) (PubMed).

Liu, Huang, Yao, Yang: "The Immunotherapeutic Role of Bacterial Lysates in a Mouse Model of Asthma." in: Lung, (2017) (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, (2016) (PubMed).

Wang, Wang, Sun, Zhang, Liu, Gadahi, Hassan, Xu, Yan, Song, Li: "Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha." in: BMC infectious diseases, Vol. 15, pp. 448, (2016) (PubMed).

Zhang, Liang, Li, Wang, Zhang, Xu, Huo, Cao, Zhong, Zhong: "Mutual enhancement of IL-2 and IL-7 on DNA vaccine immunogenicity mainly involves regulations on their receptor expression and receptor-expressing lymphocyte generation." in: Vaccine, Vol. 33, Issue 30, pp. 3480-7, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Huang, Wang, Li, Ge, Chen, Chen: "Trichostatin A reduces cisplatin-induced ototoxicity through the STAT6 signaling pathway." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 493-500, (2015) (PubMed).

Li, Hu, Wang, Wu, Zhang: "Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 64, Issue 5, pp. 363-71, (2015) (PubMed).

Qiu, Zhu, Lu, Bai, Liu, Zhao, Sun, Zhang, Li, Liu: "The selective mGluR5 agonist CHPG attenuates SO2-induced oxidative stress and inflammation through TSG-6/NF-?B pathway in BV2 microglial cells." in: Neurochemistry international, Vol. 85-86, pp. 46-52, (2015) (PubMed).

Hassan, Wang, Xu, Yan, Song, Li: "DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice." in: Parasites & vectors, Vol. 7, pp. 431, (2015) (PubMed).

Li, Han, Liang, Zhong, Li, Wen, Lin, Wang, Li, Zhong, Zhong: "Molecular mechanism for the effects of E. coli heat-labile enterotoxin on mouse embryo survival." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 31-8, (2014) (PubMed).

Ozkan, Deveci, Kumbak, Simsek, Ilhan, Sekercioglu, Sapmaz: "What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?" in: Journal of reproductive immunology, Vol. 103, pp. 53-8, (2014) (PubMed).

Ou, Shi, Xu, Tao: "Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 113, Issue 6, pp. 658-665.e1, (2014) (PubMed).

Jin, Cheng, Liu, Wu, Wang, Wei, Zhou, Qin, Jia, Zhen et al.: "Improvement of functional recovery by chronic metformin treatment is associated with enhanced alternative activation of microglia/macrophages and increased angiogenesis and neurogenesis following ..." in: Brain, behavior, and immunity, Vol. 40, pp. 131-42, (2014) (PubMed).

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Background:

Protein Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

Synonyms: Interleukin-4,IL-4,B-cell IgG differentiation factor,B-cell growth factor 1,B-cell stimulatory factor 1,BSF-1,IGG1 induction factor,Lymphocyte stimulatory factor 1,Il4,Il-4,

Full Gene Name: Interleukin-4

Cellular Localisation: Secreted.

Gene ID: 16189

UniProt: P07750

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation