CCL20 ELISA Kit

Catalog No. ABIN411326

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CCL20. There are 3 publications available.

Quick Overview for CCL20 ELISA Kit (ABIN411326)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Binding Specificity: AA 27-96

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details CCL20 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIP-3 alpha

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A27-M96

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A27-M96

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Tissue Specificity: Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

Plate: Pre-coated

Protocol: human MIP-3 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-3 alpha has been precoated onto 96-well plates. Standards(E.coli, A27-M96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-3 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIP-3 alpha amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIP-3 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MIP-3 alpha standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 94, Standard deviation: 4.42, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 325, Standard deviation: 17.55, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 592, Standard deviation: 35.52, CV(%): 6,
• Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 6.75, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 24.43, CV(%): 6.9
• Sample 3: n=24, Mean(pg/ml): 617, Standard deviation: 43.81, CV(%): 7.1

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for CCL20 ELISA Kit (ABIN411326)

Li, Wang, Liu, Meisgen, Grünler, Botusan, Narayanan, Erikci, Li, Blomqvist, Du, Pivarcsi, Sonkoly, Chowdhury, Catrina, Ståhle, Landén: "MicroRNA-132 enhances transition from inflammation to proliferation during wound healing." in: The Journal of clinical investigation, Vol. 125, Issue 8, pp. 3008-26, (2015) (PubMed).

Calvayrac, Rodríguez-Calvo, Martí-Pamies, Alonso, Ferrán, Aguiló, Crespo, Rodríguez-Sinovas, Rodríguez, Martínez-González: "NOR-1 modulates the inflammatory response of vascular smooth muscle cells by preventing NF?B activation." in: Journal of molecular and cellular cardiology, Vol. 80, pp. 34-44, (2015) (PubMed).

Chen, Hu, Mao, Jiao, Wang, Yu, Xu, Dai, Yin, Xu: "Increased IL-17-producing CD4(+) T cells in patients with esophageal cancer." in: Cellular immunology, Vol. 272, Issue 2, pp. 166-74, (2011) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20

Background:

Protein Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. .

Background: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Synonyms: C-C motif chemokine 20,Beta-chemokine exodus-1,CC chemokine LARC,Liver and activation-regulated chemokine,Macrophage inflammatory protein 3 alpha,MIP-3-alpha,Small-inducible cytokine A20,CCL20(1-67),CCL20(1-64),CCL20(2-70),CCL20,LARC, MIP3A, SCYA20,

Full Gene Name: C-C motif chemokine 20

Cellular Localisation: Secreted.

Gene ID: 6364

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection