CXCL10 ELISA Kit

Catalog No. ABIN411396

This Mouse CXCL10 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse CXCL10. This ELISA Kit has been cited in 2+ publications.

Quick Overview for CXCL10 ELISA Kit (ABIN411396)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Binding Specificity: AA 22-98

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details CXCL10 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL10/IP-10

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: I22-P98

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: I22-P98

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed in the spleen, thymus, lymph nodes and liver. .

Plate: Pre-coated

Protocol: mouse CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL10 has been precoated onto 96-well plates. Standards(E.coli, I22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL10 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CXCL10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL10 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 5.82, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 259, Standard deviation: 14.5, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 973, Standard deviation: 70.1, CV(%): 7.2,
• Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 12.25, CV(%): 9.8
• Sample 2: n=24, Mean(pg/ml): 276, Standard deviation: 22.4, CV(%): 8.1
• Sample 3: n=24, Mean(pg/ml): 1093, Standard deviation: 84.2, CV(%): 7.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for CXCL10 ELISA Kit (ABIN411396)

Tomita, Kohli, MacLaurin, Hirsova, Guo, Sanchez, Gelbard, Blaxall, Ibrahim: "Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis." in: JCI insight, Vol. 2, Issue 15, (2017) (PubMed).

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Target Details for CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: CXCL10

Background:

Protein Function: In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.

Background: Chemokine(C-X-C motif) ligand 10(CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein(gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Synonyms: C-X-C motif chemokine 10,10 kDa interferon gamma-induced protein,Gamma-IP10,IP-10,C7,Interferon-gamma induced protein CRG-2,Small-inducible cytokine B10,Cxcl10,Crg2, Ifi10, Inp10, Scyb10,

Full Gene Name: C-X-C motif chemokine 10

Cellular Localisation: Secreted.

Gene ID: 15945

UniProt: P17515