Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HMGB4 ELISA Kit

HMGB4 Reactivity: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN454716
  • Target See all HMGB4 products
    HMGB4 (High Mobility Group Box 4 (HMGB4))
    Reactivity
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156-10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Purpose
    This immunoassay kit allows for the in vitro quantitative determination of human chondroitin sulfatase,CS concentrations in cell culture supernates, serum, plasma and other biological fluids.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay recognizes recombinant and natural human CS.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference was observed.
    Sensitivity
    < 0.312 U/L
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Characteristics
    Homo sapiens,Human,High mobility group protein B4,HMGB4
    Featured
    Discover our best selling HMGB4 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
    Storage Comment
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Target See all HMGB4 products
    HMGB4 (High Mobility Group Box 4 (HMGB4))
    Alternative Name
    HMGB4 (HMGB4 Products)
    Synonyms
    HMGB4 ELISA Kit, dJ1007G16.5 ELISA Kit, 1700001F22Rik ELISA Kit, AI326135 ELISA Kit, high mobility group box 4 ELISA Kit, high-mobility group box 4 ELISA Kit, HMGB4 ELISA Kit, Hmgb4 ELISA Kit
    Background
    In enzymology, a chondroitin sulfatase is an enzyme that catalyzes the chemical reaction of cleaving off the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of the macromolecule chondroitin sulfate and, similarly, of the D-galactose 6-sulfate units of the macromolecule keratan sulfate.This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds.
You are here:
Support