CRABP2 ELISA Kit

Catalog No. ABIN455486

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CRABP2.

Quick Overview for CRABP2 ELISA Kit (ABIN455486)

Target: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312-20 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details CRABP2 ELISA Kit

Minimum Detection Limit: 0.312 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human IFN?-1b concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human IFN?-1b.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Cellular retinoic acid-binding protein 2,Cellular retinoic acid-binding protein II,CRABP-II,CRABP2

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNα-1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFNα-1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFNα-1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFNα-1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for CRABP2

Target: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Alternative Name: CRABP2