CMV IgA ELISA Kit

Catalog No. ABIN459350

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CMV IgA.

Quick Overview for CMV IgA ELISA Kit (ABIN459350)

Target: CMV IgA (Anti-Cytomegalovirus IgA Antibody (CMV IgA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details CMV IgA ELISA Kit

Purpose: anti-CMV IgA - ELISA kit for the semiquantitative detection of IgA antibodies to human cytomegalovirus (CMV) in serum or plasma.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA 8-well break-away strips coated with a mixture of specific antigens STRIPS Ag 1 x 12pcs
• 1.3 mL Positive control serum = calibrator, r.t.u. 1) CAL 1 vial
• 1.3 mL Negative control serum r.t.u. NC 1 vial
• 13 mL Anti-human IgA antibodies labelled with horseraddish peroxidase r.t.u. (Px-conjugate) CONJ 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution r.t.u. STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) (ready to use) /

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-CMV IgA is a solid-phase immunoanalytical test. The strips are coated by a mixture of specific antigens that bear immunodominant CMV epitopes. Anti-CMV antibodies, if present in the tested sera, bind to the immobilized antigens. The antibodies being in complexes with antigen are later on recognised by animal anti-human IgA antibodies labelled with horseradish peroxidase. The labelled antibodies are revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh. 8

Target Details for CMV IgA

Target: CMV IgA (Anti-Cytomegalovirus IgA Antibody (CMV IgA))

Alternative Name: Anti-Cytomegalovirus IgA Antibody

Target Type: Antibody