EBV EA(D) IgG ELISA Kit

Catalog No. ABIN459354

This Human EBV EA(D) IgG ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human EBV EA(D) IgG.

Quick Overview for EBV EA(D) IgG ELISA Kit (ABIN459354)

Target: EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details EBV EA(D) IgG ELISA Kit

Purpose: anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a specific recombinant antigen containing immunodominant epitopes of EA antigen. Anti-EA (D) antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EBV EA(D) IgG

Target: EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))

Alternative Name: Anti-EA (D) Epstein-Barr Virus (EBV) IgG Antibody

Target Type: Antibody