EBV EA(D) IgG ELISA Kit
This Human EBV EA(D) IgG ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human EBV EA(D) IgG.
Catalog No. ABIN459354
Quick Overview for EBV EA(D) IgG ELISA Kit (ABIN459354)
Target
EBV EA(D) IgG
(Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Indirect ELISA
Application
ELISA
Sample Type
Plasma, Serum
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Purpose
- anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test.
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Brand
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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Components
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control human serum, r.t.u. PC 1 vial
- 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
- 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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Material not included
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
- anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a specific recombinant antigen containing immunodominant epitopes of EA antigen. Anti-EA (D) antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Target
- EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))
Alternative Name
- Anti-EA (D) Epstein-Barr Virus (EBV) IgG Antibody
Target Type
- Antibody
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