EBV NA IgM ELISA Kit

Catalog No. ABIN459362

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EBV NA IgM.

Quick Overview for EBV NA IgM ELISA Kit (ABIN459362)

Target: EBV NA IgM (Anti-Epstein-Barr Virus Nuclear Antigen IgM (EBV NA IgM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details EBV NA IgM ELISA Kit

Purpose: anti-EBNA-1 EBV IgM is an enzyme linked imunosorbent assay.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-EBNA-1 EBV IgM is an enzyme linked imunosorbent assay. Reaction wells are coated with the nuclear antigen containing immunodominant epitopes of EBNA-1. Its immunoreactivity is increased by conjugation to a protein carrier. Anti-EBNA-1 EBV antibodies from serum samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away. If antibodies are present in the test samples, they will bind to the immobilized peptide. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgM antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EBV NA IgM

Target: EBV NA IgM (Anti-Epstein-Barr Virus Nuclear Antigen IgM (EBV NA IgM))

Target Type: Antibody