NUCB2 ELISA Kit

Catalog No. ABIN4881318

Rat, Human and Mouse NUCB2 ELISA Kit Colorimetric assay for quantification of Rat, Human and Mouse NUCB2.

Quick Overview for NUCB2 ELISA Kit (ABIN4881318)

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Reactivity: Rat, Human, Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1-1.000 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details NUCB2 ELISA Kit

Minimum Detection Limit: 0.1 ng/mL

Purpose: Human/Mouse/Rat Nucleobindin-2 EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Analytical Method: Quantitative

Specificity: This kit detects 420aa Nucleobindin-2 protein.

Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Angiotensin II, NPY and APC.

Sensitivity: 147 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Recommended Dilution for serum and plasma samplesHuman: 4X / Mouse: 4X / Rat: 2X

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for NUCB2

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Alternative Name: Nucleobindin-2

Gene ID: 4925

UniProt: P80303