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Resistin ELISA Kit

Horse Resistin ELISA Kit Colorimetric assay for quantification of Horse Resistin.
Catalog No. ABIN4881615

Quick Overview for Resistin ELISA Kit (ABIN4881615)

Target

See all Resistin (RETN) ELISA Kits
Resistin (RETN)

Reactivity

  • 8
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Horse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

46-3000 pg/mL

Application

ELISA

Sample Type

Plasma, Cell Culture Supernatant, Serum
  • Minimum Detection Limit

    46 pg/mL

    Purpose

    Equine Resistin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

    Analytical Method

    Quantitative

    Specificity

    This ELISA antibody pair recognizes Equine Resistin.

    Characteristics

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Components

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Material not included

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes

    Recommended Dilution for serum and plasma samples10 fold

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Protocol

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Restrictions

    For Research Use only
  • Storage

    -20 °C

    Storage Comment

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Expiry Date

    6 months
  • Target See all Resistin (RETN) ELISA Kits

    Resistin (RETN)

    Alternative Name

    Resistin

    UniProt

    F6W1S0

    Pathways

    Feeding Behaviour, Smooth Muscle Cell Migration
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