Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Kallikrein 8 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Kallikrein 8 in Cell Culture Supernatant, Cell Lysate, Plasma, Serum and Tissue Lysate.
Catalog No. ABIN4883542

Quick Overview for Kallikrein 8 ELISA Kit (ABIN4883542)

Target

See all Kallikrein 8 (KLK8) ELISA Kits
Kallikrein 8 (KLK8)

Reactivity

  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • Purpose

    Custom Human Kallikrein 8 (Neuropsin) ELISA Kit.

    Analytical Method

    Quantitative

    Characteristics

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Components

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Material not included

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume

    100 μL

    Plate

    Pre-coated

    Protocol

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Restrictions

    For Research Use only
  • Storage

    -20 °C

    Storage Comment

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Expiry Date

    6 months
  • Target See all Kallikrein 8 (KLK8) ELISA Kits

    Kallikrein 8 (KLK8)

    Alternative Name

    Kallikrein 8

    Gene ID

    11202

    UniProt

    O60259

    Pathways

    Complement System
You are here:
Chat with us!