IL12 ELISA Kit (Interleukin 12)

Details for Product IL12 ELISA Kit No. ABIN4885562
Antigen
  • CLMF
  • CLMF2
  • IL-12B
  • NKSF
  • NKSF2
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • IL-12p40
  • IL-12
  • LOC100217488
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • IL12p40
  • IL12B
  • IL-12 p35
  • interleukin 12B
  • interleukin 12A
  • interleukin 12b
  • interleukin 12a
  • Interleukin 12 level
  • IL12B
  • IL12A
  • Il12b
  • Il12a
  • IL12
  • il12a
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
9
5
5
5
5
4
3
3
3
3
3
2
2
2
1
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
5-2000 pg/mL
Minimum Detection Limit
5 pg/mL
Application
ELISA
Options
Purpose Mouse IL-12 p40/p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity 5 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others IL12 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name IL-12 p40/70 (IL12 ELISA Kit Abstract)
Background Gene Names: Il12b
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
Gene ID 16160
UniProt P43432
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints
Application Notes Recommended Dilution for serum and plasma samples10 - 100 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
Image no. 1 for Interleukin 12 (IL12) ELISA Kit (ABIN4885562) Interleukin 12 (IL12) ELISA Kit
Product cited in: Shanmugam, Wang, Markand, Perry, Tawfik, Zorrilla, Ganapathy, Smith: "Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." in: Journal of neurochemistry, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: Conditioned Media (Muller cells incubated with LPS)

Spreadbury, Ochoa-Cortes, Ibeakanma, Martin, Hurlbut, Vanner: "Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 27, Issue 3, pp. 347-55, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: Conditioned Media (Soluble colonic mediators from isolated colon tissues)

Ghosh, Mukherjee, Choudhury, Gupta, Adhikary, Baral, Chattopadhyay: "Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine." in: Cellular signalling, Vol. 27, Issue 7, pp. 1398-412, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: Conditioned Media (Swiss Albino mice peritoneal macrophages)

Bishayi, Bandyopadhyay, Majhi, Adhikary: "Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophages and possible mechanisms of CCR-2/TLR-2 and MCP-1 signalling during Staphylococcus aureus infection." in: Immunobiology, Vol. 220, Issue 3, pp. 350-62, 2015 (PubMed).

Abo Elnazar, Ghazy, Ghoneim, Taha, Abouelella: "Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes." in: Frontiers in pharmacology, Vol. 6, pp. 56, 2015 (PubMed).

Ghazy, Abu El-Nazar, Ghoneim, Taha, Abouelella: "Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes." in: Frontiers in pharmacology, Vol. 6, pp. 74, 2015 (PubMed).

Fulzele, Krause, Panaroni, Saini, Barry, Liu, Lotinun, Baron, Bonewald, Feng, Chen, Weinstein, Wu, Kronenberg, Scadden, Divieti Pajevic: "Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling." in: Blood, Vol. 121, Issue 6, pp. 930-9, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: Conditioned Media (osteocyte-enriched bone explants)

Matsushita, Seike, Okawa, Kadawaki, Ohtsu: "Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis." in: Experimental dermatology, Vol. 21, Issue 9, pp. 714-5, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: Tissue Lysate (Skin lysates from trinotriochlorobenzene TNCB treatment, allergic dermatitis model)

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