GM-CSF ELISA Kit

Catalog No. ABIN4986886

Mouse GM-CSF ELISA Kit Colorimetric assay for quantification of Mouse GM-CSF.

Quick Overview for GM-CSF ELISA Kit (ABIN4986886)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.03-2.0 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details GM-CSF ELISA Kit

Minimum Detection Limit: 0.03 ng/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Mouse GM-CSF Ligand

Sensitivity: 15 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: Granulocyte macrophage colony-stimulating factor (GM-CSF), a 22 kDa glycosylated protein, was initially characterized as a factor that can support the in vitro colony formation of granulocytemacrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3 5). It shows clinical effectiveness in ameliorating chemotherapyinduced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). Mature mouse GM-CSF shares 49 % -54 % amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69 % with rat GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF Rα/CD116 and the signal transducing common β chain (CD131) which is also a component of the highaffinity receptors for IL-3 and IL-5 (8,9). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Rα (10). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (11).

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin