Quick Overview for IgE ELISA Kit (ABIN4986922)
Target
See all IgE ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.31 ng/mL
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Analytical Method
- Quantitative
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Specificity
- Natural and recombinant Mouse IgE Ligand
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Sensitivity
- 156 pg/mL
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Material not included
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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IgE ELISA Kit
Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
IgE ELISA KitReactivity: Rabbit Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
IgE ELISA KitReactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgE ELISA KitReactivity: Human Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Urine
IgE ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgE ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 125 ng/mL - 8000 ng/mL Cell Culture Supernatant, Plasma, Serum
IgE ELISA KitReactivity: Dog Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgE ELISA KitReactivity: Cat Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgE ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 1 ng/mL - 32 ng/mL Plasma, Serum, Urine
IgE ELISA KitReactivity: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Detection Wavelength: 450 nm
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Sample Volume
- 20 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IgE
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Background
- There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgM. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disulfide bridges of cysteine residues. Mouse IgE, also known as reaginic antibody, exists in serum as a 4-chain monomer of 185-200 kDa. Like IgM, the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 μg/mL. IgE is cyolytic for mast cells, where it binds to the Fc?RI receptor and is able to mediate the response of granular and lipid mediators of inflammation, including the PCA reaction. The IgE class switch is induced by IL-4 as part of the Th2 response.
Target See all IgE ELISA Kits
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