IL-10 ELISA Kit

Catalog No. ABIN4986925

Rat IL-10 ELISA Kit Colorimetric assay for quantification of Rat IL-10.

Quick Overview for IL-10 ELISA Kit (ABIN4986925)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.03-2.0 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL-10 ELISA Kit

Minimum Detection Limit: 0.03 ng/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Rat IL-10 Ligand

Sensitivity: 15 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Background: Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitor, is a pleiotropic cytokine that inhibits the production of a number of cytokines (including IL-1, GM-CSF, TNF, IL-6, IL-8, IL-12 and IFN-γ) by activated Th1 cells, NK cells, and monocytes/macrophages(1-5). Rat IL-10 cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein (1, 2). Within the mature protein, there are two potential N-linked glycosylation sites and five cysteine residues. Mature rat IL-10 shares 85 % and 74 % aa sequence identity with mouse and human IL-10, respectively (1, 4). Although rat and human IL-10 are both active on mouse cells, mouse IL-10 is not active on human cells (4).Based on human and mouse studies, biologically active rat IL-10 is likely to be a nondisulfide-linked homodimer in solution (4). Although IL-10 is considered to be a strictly secreted cytokine, the existence of biologically-active, membrane-bound IL-10 has also been reported. IL-10 has been shown to inhibit macrophage cytotoxic activity and to stimulate the proliferation and differentiation of B cells, mast cells, and thymic T cells. IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 (6, 7). Finally, IL-10 has been reported to suppress the synthesis of superoxide anion (8) plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages (9). IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 (10), and on thymocytes (11) and mast cells, where it acts as a growth costimulator (12, 13).

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints