IL1A ELISA Kit

Catalog No. ABIN4986937

Mouse IL1A ELISA Kit Colorimetric assay for quantification of Mouse IL1A.

Quick Overview for IL1A ELISA Kit (ABIN4986937)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.8-125 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL1A ELISA Kit

Minimum Detection Limit: 1.8 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Mouse IL-1a Ligand

Sensitivity: 1 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1a

Background: Interleukin-1 alpha(IL-1a) is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. IL-1 alpha is synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. The IL-1a precursor reportedly shows full biological activity in the EL-4 assay. Circulating levels of IL-1a are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1a possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints