IL-5 ELISA Kit

Catalog No. ABIN4986959

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-5.

Quick Overview for IL-5 ELISA Kit (ABIN4986959)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL-5 ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human IL-5 Ligand

Sensitivity: 2 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5

Background: Interleukin-5(IL-5)is a secreted glycoprotein that belongs to the α-helical group of cytokines (1-3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70 % , 70 %, 62 %, 71 %, 70 % and 66 %, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross-reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour