IL-9 ELISA Kit

Catalog No. ABIN4986965

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-9.

Quick Overview for IL-9 ELISA Kit (ABIN4986965)

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL-9 ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human IL-9 Ligand

Sensitivity: 2 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-9

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Alternative Name: IL-9

Background: Human IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLV-I) transformed T cell line that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, M07e. This human cytokine and its murine homologue are now designated as human and mouse IL-9. The gene for H IL-9 has been mapped to human chromosome 5. As in the mouse system, the human IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteinerich protein with a predicted molecular mass of 14 kDa. Human IL-9 contains four potential Nlinked glycosylation sites and the native HIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56 % and 67 % homology at the amino acid and nucleotide levels, respectively. Although murine IL-9 is active on human cells, human IL-9 is not active on mouse cells. Human and murine IL9 are also capable of enhancing in vitro survival of human T cell lines as well as synergizing with Epo to support erythroid colony formation in vitro.

Pathways: JAK-STAT Signaling