Leptin ELISA Kit

Catalog No. ABIN4987005

This Human Leptin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Leptin.

Quick Overview for Leptin ELISA Kit (ABIN4987005)

Target: Leptin (LEP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details Leptin ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinantHuman Leptin Ligand

Sensitivity: 7 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Human Leptin (gene name OB) is a 16 kDa, 146 amino acid (aa) residue, non-glycosylated polypeptide that regulates adipose tissue mass and energy balance (1 - 6). Mature human Leptin shares 87 % and 84 % aa identity with mouse and rat Leptin, respectively(1,7). Human Leptin is active in both the mouse and rat systems (8, 9). Leptin is expressed almost exclusively by adipocytes and its production is influenced by hormones, cytokines and nutrients (5, 7, 10) and circulates in the plasma, crosses the blood-brain barrier, and is present in human breast milk (3 - 6, 11). The human Leptin receptor (designated ObR or LEPR) is a 150 kDa, 1144 aa residue, type I transmembrane glycoprotein of the IL-6 receptor family of Class I cytokine receptors (12, 13). The gene for ObR undergoes considerable splicing, forming variants a-d with cytoplasmic domains of variable length, plus the potentially soluble form ObRe (13, 14). The long form, ObRb (formerly OB RL), is expressed mainly in the hypothalamic arcuate nucleus and is essential for signal transduction (6, 15, 16). In a concentration-dependent manner, Leptin signaling can have diverse effects, causing neurons that express pro-opiomelanocortin (POMC) peptides to reduce food intake, and neurons that express neuropeptide Y and agouti-related protein (NpY and AgRP) to increase food intake (4, 6). Leptin is fundamentally a

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process