NAMPT ELISA Kit

Catalog No. ABIN4987044

This Human NAMPT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human NAMPT.

Quick Overview for NAMPT ELISA Kit (ABIN4987044)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details NAMPT ELISA Kit

Minimum Detection Limit: 1.56 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human PBEF/ Visfatin Ligand

Sensitivity: 0.70 ng/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: PBEF/ Visfatin

Background: PBEF (preB cell colonyenhancing factor? also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the Cterminal 128 amino acids (aa 364491). Over aa 27491, human PBEF shares 96 % , 97 %, and 96 % aa identity with mouse, porcine, and canine PBEF, respectively.