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IgG2a ELISA Kit

Rat IgG2a ELISA Kit Colorimetric assay for quantification of Rat IgG2a.
Catalog No. ABIN4993803

Quick Overview for IgG2a ELISA Kit (ABIN4993803)

Target

See all IgG2a products
IgG2a

Reactivity

  • 3
  • 2
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.78-50 ng/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma, Serum
  • Minimum Detection Limit

    0.78 ng/mL

    Purpose

    This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IgG2a.

    Analytical Method

    Quantitative

    Specificity

    IgG2a

    Components

    plate, standard, diluent

    Material not included

    pipettes, tubes, reader
  • Sample Volume

    100 μL

    Assay Time

    3 - 4 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Buffer

    0.02 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C
  • Target See all IgG2a products

    IgG2a

    Target Type

    Antibody

    Background

    Antibody isotyping is a critical and beneficial aspect of hybridoma development. This kit can identify six immunoglobulin heavy chain isotypes and two light chain isotypes in mice: IgA, IgM, IgG1, IgG2a, IgG2b, IgG3, kappa chain and lambda chain. It can accurately and specifically identify which heavy and light chain of the hybridoma samples is producing and if it is monoclonal or not. This kit is a powerful tool to isolate and characterize each potential clone.
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