IL-3 ELISA Kit

Catalog No. ABIN5026937

This Human IL-3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-3.

Quick Overview for IL-3 ELISA Kit (ABIN5026937)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-3 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-3 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensitivity: < 9.4 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human IL-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-3bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3 (Interleukin 3)

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy