IL-7 ELISA Kit

Catalog No. ABIN5026943

This Human IL-7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-7.

Quick Overview for IL-7 ELISA Kit (ABIN5026943)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-7 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-7 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensitivity: < 4.69 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-humanIL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-7antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7 (Interleukin 7)

Pathways: JAK-STAT Signaling