CCL3 ELISA Kit
Quick Overview for CCL3 ELISA Kit (ABIN5026947)
Target
See all CCL3 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 23.44 pg/mL
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Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human MIP-1Alpha /CCL3 concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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Sensitivity
- < 14 pg/mL
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Components
- plate, standard, diluent, antibodies
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Material not included
- pipettes, tubes, reader, incubator
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Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Dog Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Rat Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Mouse AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Rat AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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Sample Volume
- 100 μL
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Assay Time
- 3 - 4 h
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Plate
- Pre-coated
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Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MIP-1Alpha /CCL3 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MIP-1Alpha /CCL3 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1Alpha /CCL3 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MIP-1Alpha /CCL3 can be calculated using the standard curve.
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Restrictions
- For Research Use only
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Precaution of Use
- protect your eyes
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Storage
- 4 °C
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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Alternative Name
- MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha)
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
Target See all CCL3 ELISA Kits
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