FGF2 ELISA Kit

Catalog No. ABIN5067895

This Colorimetric ELISA kit is designed for the quantitative measurement of Human FGF2.

Quick Overview for FGF2 ELISA Kit (ABIN5067895)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Lysate, Plasma, Serum

Product Details FGF2 ELISA Kit

Purpose: Human bFGF ELISA Kit is for the quantitative determination of human basic fibroblast growth factor (bFGF) concentrations in serum, plasma, cell culture supernatant, urine, and other biological fluids.

Analytical Method: Quantitative

Sensitivity: 80 pg/mL

Characteristics: Fibroblast Growth Factor, Basic, Human (FGFb) ELISA Kit

Components:

• 1. bFGF MICROTITER PLATE, 1x96 wells
• 2. BIOTIN CONJUGATE, 1x7 mL
• 3. AVIDIN CONJUGATE, 1x14 mL
• 4. bFGF STANDARD, 2x1 vials
• 5. CALIBRATOR DILUENT I, 1x 22 mL For serum/plasma testing.
• 6. CALIBRATOR DILUENT II, 1x 22 mL For cell culture supernatant/urine testing.
• 7. WASH BUFFER (20X) , 1x 60 mL
• 8. SUBSTRATE A, 1x 11 mL
• 9. SUBSTRATE B , 1x11 mL
• 10. STOP SOLUTION , 1x14 mL

Application Details

Protocol: Principle:

• The bFGF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for bFGF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. bFGF if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound bFGF and other components of sample. In order to quantitative the amount of bFGF present in the sample, a standardized preparation of horseradish peroxidase HRP)-conjugated polyclonal antibody specific for bFGF is added to each well to "sandwich" the bFGF immobilised during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3&
• 39,5,5&
• 39, tetramethyl-benzidine) substrate solution is added to each well.
• The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain bFGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured by spectrophotometer at a wavelength of 450nm ± 2nm.
• In order to measure the concentration of bFGF in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2- fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus bFGF concentration (pg/mL). The concentration of bFGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-80 °C

Storage Comment: 4°C/-70°C

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: Fibroblast Growth Factor basic

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins