For best experience we recommend to activate Javascript in your browser.

Resistin ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Resistin.

Catalog No. ABIN5067898

Datasheet as PDF

Quick Overview for Resistin ELISA Kit (ABIN5067898)

Target

See all Resistin (RETN) ELISA Kits

Resistin (RETN)

Reactivity

Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Application

ELISA

Sample Type

Plasma, Serum, Tissue Culture Medium

Discover our top product RETN ELISA Kit

Purpose

Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.

Analytical Method

Quantitative

Sensitivity

0.012 ng/mL

Characteristics

Resistin, Human, ELISA Kit

Components
- Microtiter Plate, 1x96wells
- Standard, 1x1vial
- High Control, 1x1vial
- Low Control, 1x1vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Dilution Buffer, 1x10ml
- Wash Solution (10X), 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (O4), 1x13ml
Resistin (RETN) ELISA Kit
RETN Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum

Resistin (RETN) ELISA Kit
RETN Reactivity: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Monkey Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Sheep Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Rabbit Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Resistin (RETN) ELISA Kit
RETN Reactivity: Sheep Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum

Resistin (RETN) ELISA Kit
RETN Reactivity: Human AA 19-108 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Protocol

Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.

Restrictions

For Research Use only
Storage

4 °C

Storage Comment

4°C
Target See all Resistin (RETN) ELISA Kits

Resistin (RETN)

Alternative Name

Resistin

Pathways

Feeding Behaviour, Smooth Muscle Cell Migration