Resistin ELISA Kit
Quick Overview for Resistin ELISA Kit (ABIN5067898)
Target
See all Resistin (RETN) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
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Analytical Method
- Quantitative
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Sensitivity
- 0.012 ng/mL
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Characteristics
- Resistin, Human, ELISA Kit
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Components
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- Microtiter Plate, 1x96wells
- Standard, 1x1vial
- High Control, 1x1vial
- Low Control, 1x1vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Dilution Buffer, 1x10ml
- Wash Solution (10X), 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (O4), 1x13ml
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Resistin (RETN) ELISA Kit
RETN Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Resistin (RETN) ELISA KitRETN Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Monkey Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Sheep Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Rabbit Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN Reactivity: Human AA 19-108 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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Protocol
- Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C
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- Resistin (RETN)
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Alternative Name
- Resistin
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Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
Target See all Resistin (RETN) ELISA Kits
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