Transferrin Receptor ELISA Kit

Catalog No. ABIN5067899

This Human Transferrin Receptor ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Transferrin Receptor.

Quick Overview for Transferrin Receptor ELISA Kit (ABIN5067899)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Lysate, Plasma, Serum

Product Details Transferrin Receptor ELISA Kit

Purpose: Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.

Analytical Method: Quantitative

Sensitivity: 2 ng/mL

Characteristics: Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)

Components:

• Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
• Mab (HRP), 1x13ml
• Standard, 2ug/ml, 1x100ul
• Standard, 1ug/ml, 1x100ul
• Standard, 0.5ug/ml, 1x.100ul
• Standard, 0.2ug/ml, 1x100ul
• Standard, 0.1ug/ml, 1x100ul
• Standard, 0.05ug/ml, 1x100ul
• Quality Control, High, 50X, (5..3ug /ml), 1x50ul
• Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
• Dilution Buffer, 2x13ml
• Wash Solution, 10X, 1x100ml
• Substrate Solution (TMB), 1x13ml
• Stop Solution (0.2M O4), 1x13ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: Transferrin Receptor

Pathways: Transition Metal Ion Homeostasis