Pepsin ELISA Kit
Quick Overview for Pepsin ELISA Kit (ABIN5067908)
Target
See all Pepsin ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- PP (Pepsin) ELISA Kit is for the quantitative detection of Pepsin in porcine serum, plasma, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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Sensitivity
- 0.938 ng/mL
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Characteristics
- PP (Pepsin) ELISA Kit (Porcine)
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Components
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- *358254A: Microtiter Strips, 1x96 wells (8x12 wells).
- *358254B: Standard, 2x1 vial
- 358254C: Sample/Standard Dilution Buffer, 1x20ml
- 358254D: Antibody (Biotin) (Concentrated), 1x120ul
- 358254E: Antibody Dilution Buffer, 1x10ml
- 358254F: Streptavidin (HRP) (SABC), 1x120ul
- 358254G: SABC Dilution Buffer, 1x10ml
- 358254H: TMB Substrate, 1x10ml
- 358254J: Stop Solution, 1x10ml
- 358254K: Wash Buffer, 25X, 1x30ml
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Pepsin ELISA Kit
Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Pepsin ELISA KitReactivity: Human Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Pepsin ELISA KitReactivity: Rat Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReactivity: Mouse Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitInstant Reactivity: Human Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitSmall Sample Reactivity: Mouse Colorimetric Sandwich ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
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Plate
- Pre-coated
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Protocol
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Principle:
- This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to Pepsin. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain Pepsin, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pepsin in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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Assay Precision
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C/-20°C
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- Pepsin
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Alternative Name
- PP (Pepsin)
Target See all Pepsin ELISA Kits
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