RBP4 ELISA Kit
Quick Overview for RBP4 ELISA Kit (ABIN5067911)
Target
See all RBP4 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- Retinol Binding Protein 4 ELISA Kit (Human) (RBP4) is for the quantitative determination of human Retinol-Binding Protein 4 (RBP4) concentrations in cell culture supernates, serum, plasma, urine, and saliva.
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Analytical Method
- Quantitative
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Sensitivity
- 0.628 ng/mL
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Characteristics
- Retinol Binding Protein 4 ELISA Kit (Human) (RBP4)
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Components
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- Microtiter Strips 1x96 wells
- Mab (HRP) Conjugate, 1x21ml
- Standard (lyophilized), 1x200ng
- Assay Diluent, 2x11ml
- Calibrator Diluent, 1x21ml
- Wash Buffer Concentrate (25X), 1x 21ml
- Color Reagent A, (2) 1x12ml
- Color Reagent B (TMB), 1x12ml
- Stop Solution (2N O4) 1x6ml
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Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
RBP4 Reactivity: Human Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Rat Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Chicken Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Human AA 19-201 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 Reactivity: Human Colorimetric Competition ELISA 15.6-1000 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
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Principle of the Assay:
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for RBP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBP4 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for RBP4 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C
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- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
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Alternative Name
- Retinol Binding Protein 4
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Pathways
- Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
Target See all RBP4 ELISA Kits
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