beta Amyloid ELISA Kit

Catalog No. ABIN5067940

This Colorimetric ELISA kit is designed for the quantitative measurement of Human beta Amyloid.

Quick Overview for beta Amyloid ELISA Kit (ABIN5067940)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Binding Specificity: AA 1-40

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cerebrospinal Fluid, Tissue Homogenate, Tissue Culture Medium

Product Details beta Amyloid ELISA Kit

Purpose: The Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.

Analytical Method: Quantitative

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Sensitivity: > 6 pg/mL

Characteristics: β-Amyloid, aa1-40, Human, ELISA Kit

Components:

• * beta Amyloid Standard, 1x1vial
• Standard Diluent Buffer, 1x60ml
• Microtiter Plate, 1x96 wells
• beta Amyloid , Pab 1x6ml
• IgG (HRP) (100X), 1x125ul
• HRP Diluent, 1x25ml
• Wash Buffer (25X), 1x100ml
• TMB, 1x25ml
• Stop Solution, 1x25ml

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C,-80 °C

Storage Comment: 4°C/-70°C

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: beta-Amyloid

Pathways: Inflammasome