Defensin beta 3 ELISA Kit

Catalog No. ABIN5067941

This Human Defensin beta 3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Defensin beta 3.

Quick Overview for Defensin beta 3 ELISA Kit (ABIN5067941)

Target: Defensin beta 3 (DEFB3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5-400 pg/mL

Application: ELISA

Sample Type: Serum, Cell Culture Samples, Saliva, Tissue Samples

Product Details Defensin beta 3 ELISA Kit

Minimum Detection Limit: 12.5 pg/mL

Purpose: The Human Beta Defensin 3 (BD-3) ELISA Kit is an in vitro immunoassay for research use in the quantification of BD-3 in cultures of human cells and in appropriately qualified samples from serum, saliva, or other tissue fluids. The kit is suitable for testing a variety of sample types, in accordance with appropriate validation of linearity and recovery.

Analytical Method: Quantitative

Sensitivity: 20 pg/mL

Characteristics: Beta Defensin 3, Human, ELISA Kit (BD3)

Components:

• Microwell Strips, 1x96 wells (12x8-well strips). Coated with purified anti-human BD3 antibodies. Return unused strips to the pouch with desiccant, re-seal and store refrigerated.
• Standard, 400pg/ml, 2x1 vial. Recombinant human BD3. Supplied as a lyophilized powder from buffer, protein stabilizers.
• Ab (Biotin), 100X, 1x150ul. Biotinylated anti-human BD3. Supplied as a liquid in buffer, protein, detergents, 0.05% Bronidox.
• Streptavidin (HRP), 100X, 1x150ul. Supplied as a liquid in buffer with protein, detergents, 0.05% Bronidox.
• Sample Diluent, 10X, 1x10ml.
• Biotin, HRP Diluent, 1x22ml. Supplied as a liquid in buffer, protein, anti-microbials.
• Wash Solution, 100X, 1x10ml.
• TMB Solution, 1x12ml. Chromogenic substrate for HRP containing TMB and peroxide.
• Stop Solution (1% O4), 1x12ml. 1% sulfuric acid. Caution: Corrosive!

Application Details

Plate: Pre-coated

Protocol: Principle:

• The assay is based on the binding of human Beta Defensin 3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to biotin, which then binds to a streptavidin (HRP). After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of BD-3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of BD-3 in samples is calculated from a standard curve of purified recombinant human BD-3 of designated concentration.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Defensin beta 3

Target: Defensin beta 3 (DEFB3)

Alternative Name: Beta Defensin 3

Pathways: Production of Molecular Mediator of Immune Response