EGF ELISA Kit

Catalog No. ABIN5067954

Human EGF ELISA Kit Colorimetric assay for quantification of Human EGF.

Quick Overview for EGF ELISA Kit (ABIN5067954)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Lysate, Plasma, Serum, Urine

Product Details EGF ELISA Kit

Purpose: The Human Epidermal Growth Factor (Hu EGF) ELISA is to be used for the in vitro quantitative determination of human EGF in human serum, plasma, buffered solution, cell culture medium or urine. The assay will recognize both natural and recombinant human EGF.

Analytical Method: Quantitative

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Sensitivity: > 1 pg/mL

Characteristics: Epidermal Growth Factor, Human (EGF) ELISA Kit

Components:

• * Hu EGF Standard, purified recombinant Hu EGF expressed in E. coli. Refer to vial label for quantity and reconstitution volume. 2vials, 4 vials
• Standard Diluent Buffer 15mM sodium azide 1x25ml, 2x25ml
• Plate Covers, adhesive strips.
• Hu EGF Antibody-Coated Wells, 96 wells per plate. 1plate, 2plates
• Hu EGF Biotin Conjugate (Biotin-labeled anti-EGF). Contains 15 mM sodium azide, 1x11ml, 2x11ml
• Streptavidin-Peroxidase (HRP), (100x) concentrate. Contains 3.3 mM thymol, 1x0.125ml, 2x125ml
• Streptavidin-Peroxidase (HRP) Diluent. Contains 3.3mM thymol, 1x25ml
• Wash Buffer Concentrate (25x), 1x100ml
• Stabilized Chromogen, Tetramethylbenzidine (TMB) 1x25ml
• Stop Solution 1x25ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The human EGF kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A polyclonal antibody specific for human EGF has been coated onto the wells of the microtiter
• strips provided. Samples, including standards of known human EGF content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the Hu EGF antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for Hu EGF is added. During the second incubation, this antibody binds to the immobilized Hu EGF captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to
• remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu EGF present in the original specimen.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C Do not freeze

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Epidermal Growth Factor

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation