IgG1 ELISA Kit
Quick Overview for IgG1 ELISA Kit (ABIN5067980)
Target
See all IgG1 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The IgG1 Murine BioAssay ELISA Kit is an in vitro immunoassay for the quantification of IgG1 circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.
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Analytical Method
- Quantitative
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Sensitivity
- 5 ng/mL
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Characteristics
- IgG1, Mouse, ELISA Kit
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Components
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- Microtiter Plate: 1x96 wells
- Positive Control (ng/ml): 1x650ul
- Standard 5ng/ml, 1x650ul
- Standard 10ng/ml, 1x650ul
- Standard 20ng/ml, 1x650ul
- Standard 40ng/ml, 1x650ul
- Standard 100ng/ml, 1x650ul
- IgG1 (HRP), 100X, 1x150ul
- Sample Diluent, 20X, 1x10ml
- Wash Solution, 100X, 1x10ml
- TMB Substrate, 1x12ml
- Stop Solution, 1x12ml
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IgG1 ELISA Kit
Reactivity: Human Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG1 ELISA KitReactivity: Mouse Colorimetric Competition ELISA 617.28 ng/mL - 50000 ng/mL Plasma, Serum
IgG1 ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
IgG1 ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgG1 ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
IgG1 ELISA KitReactivity: Sheep Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
IgG1 ELISA KitReactivity: Guinea Pig Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
IgG1 ELISA KitReactivity: Rat Colorimetric Competition ELISA 49.4 ng/mL - 4000 ng/mL Plasma, Serum
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Protocol
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Principle:
- The Mouse IgG1 ELISA kit is based on the binding of mouse IgG1 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG1 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG1 in samples and control is calculated from a curve of standards containing known concentrations of IgG1.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C
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- IgG1
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Target Type
- Antibody
Target See all IgG1 ELISA Kits
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