FGF2 ELISA Kit

Catalog No. ABIN5068059

Human FGF2 ELISA Kit Colorimetric assay for quantification of Human FGF2.

Quick Overview for FGF2 ELISA Kit (ABIN5068059)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Serum

Product Details FGF2 ELISA Kit

Purpose: This Human Fibroblast Growth Factor basic (FGF-b) ELISA kit is to be used for the quantitative determination of FGF basic in human serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18kD isoform and recombinant FGF basic-146 and FGF basic-157.

Analytical Method: Quantitative

Sensitivity: 7 pg/mL

Characteristics: Fibroblast Growth Factor, Basic, Human (FGFb) ELISA Kit

Components:

• Microtiter Plate, 1x96wells. FGFb antibody-coated wells.
• Standard 2x1vial.
• Standard Diluent, 1x25ml. Contains 0.1% sodium azide.
• FGFb (Biotin) Pab, 1x11ml. Contains 0.1% sodium azide.
• Streptavidin (HRP), 100X, 1x125ul. Contains 3.3mM thymol.
• Streptavidin-HRP Diluent, 1x25ml. Contains 3.3mM thymol.
• Wash Buffer Concentrate, 25X, 1x100ml.
• TMB, 1x25ml.
• Stop Solution, 1x25ml.

Application Details

Plate: Pre-coated

Protocol: Principle of the Method:

• This human FGF basic kit is a solid phase sandwich ELISA Assay. A monoclonal antibody specific for human FGF basic has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human FGF-b content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the FGF-b antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for FGF-b is added. During the second incubation, this antibody binds to the immobilized FGF-b captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of FGF-b present in the original specimen.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: Fibroblast Growth Factor basic

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins