beta Catenin ELISA Kit

Catalog No. ABIN5068366

This Colorimetric ELISA kit is designed for the quantitative measurement of Human beta Catenin.

Quick Overview for beta Catenin ELISA Kit (ABIN5068366)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate

Product Details beta Catenin ELISA Kit

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Characteristics: Catenin, beta, Human, ELISA Kit

Components:

• 1. Microtiter Plate, Catenin antibody-coated microtiter, 1x96 wells.
• 2. Beta-Catenin Detection Ab, 1x11ml
• 3. IgG (HRP) Ab, 1x11ml
• 4. TMB Substrate, 1x 11ml
• 5. STOP Solution, 1x11ml
• 6. Wash Buffer (20X), 1x25ml
• 7. Sample Diluent, 1x25ml.
• 8. Cell Lysis Buffer, 1x15ml

Application Details

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: -20°C

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Catenin, beta

Background: A key downstream effector in the Wnt signaling pathway is b-catenin (1). It is implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). Casein kinase 1 phosphorylates b-catenin on Ser45. This phosphorylation event primes b-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3b destabilizes b-catenin by phosphorylating it at Ser33, 37 and Thr41 (7). Mutations in these phosphorylation sites which result in the stabilization of b-catenin protein levels have been found in many tumor cell lines (8).

Pathways: Peptide Hormone Metabolism