Rheumatoid Factor ELISA Kit

Catalog No. ABIN5068389

Mouse Rheumatoid Factor ELISA Kit Colorimetric assay for quantification of Mouse Rheumatoid Factor.

Quick Overview for Rheumatoid Factor ELISA Kit (ABIN5068389)

Target: Rheumatoid Factor (RF)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum

Product Details Rheumatoid Factor ELISA Kit

Characteristics: Rheumatoid Factor (RF), Mouse, ELISA Kit

Components:

• Microwell Strips, 1x96 wells
• Negative Control, 1x1ml
• Positive Control, 1x1ml
• Sample Diluent, 10X, 1x10ml
• IgG (HRP), 100X GtxMo, 1x200ul
• Wash Buffer, 100X, 1x10ml
• TMB Substrate, 1x12ml
• Stop Solution. 1x12ml

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Rheumatoid Factor

Target: Rheumatoid Factor (RF)

Background: Rheumatoid Factors (RFs) were described as Immunoglobulins (IgGs) in rheumatoid arthritis as detected by agglutination of rabbit IgG coated erythrocytes. It was later demonstrated that RFs were IgM molecules reactive with the Fc-portion of the IgG from various species. In addition to the IgM RF, subsequent studies have identified RFs of other IgG subtypes (IgG, IgA, and IgE) in sera of patients with rheumatoid arthritis, and certain other chronic diseases. Most commercial assays measure predominantly IgM RFs. It has been observed that measurement of IgG RF may be a better indicator of rheumatoid arthritis than IgM RF. IgA RF and IgE RF have been implicated in articular disease process in rheumatoid arthritis and rheumatoid vasculitis, respectively. RFs, produced by cells within the joint space, are found in joint fluids as well. RF titer, particularly IgG RF correlate with the intensity of synovitis.