GFP ELISA Kit

Catalog No. ABIN5510638

This Colorimetric ELISA kit is designed for the quantitative measurement of Aequorea victoria GFP.

Quick Overview for GFP ELISA Kit (ABIN5510638)

Target: GFP (Green Fluorescent Protein (GFP))

Binding Specificity: AA 2-238

Reactivity: Aequorea victoria

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details GFP ELISA Kit

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of GFP

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S2-K238

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Characteristics:

Tissue Specificity: Photocytes.

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Protein Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Background: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.

Synonyms: Green fluorescent protein,GFP,

Full Gene Name: Green fluorescent protein

UniProt: P42212