SPHK1 ELISA Kit

Catalog No. ABIN5510666

This Human SPHK1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human SPHK1.

Quick Overview for SPHK1 ELISA Kit (ABIN5510666)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Binding Specificity: AA 1-384

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details SPHK1 ELISA Kit

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SPHK1

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: M1-L384

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Characteristics:

Tissue Specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. .

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background:

Protein Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. .

Background: Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms: Sphingosine kinase 1,SK 1,SPK 1,2.7.1.91,SPHK1,SPHK, SPK,

Full Gene Name: Sphingosine kinase 1

Cellular Localisation: Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.

UniProt: Q9NYA1

Pathways: VEGF Signaling