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IgA ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog IgA.
Catalog No. ABIN5519875

Quick Overview for IgA ELISA Kit (ABIN5519875)

Target

See all IgA ELISA Kits
IgA

Reactivity

  • 9
  • 8
  • 6
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Dog

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.25 pg/mL - 2000 pg/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    31.25 pg/mL

    Purpose

    For quantitative detection of IgA in serum, plasma, tissue homogenates.

    Analytical Method

    Quantitative

    Specificity

    This assay has high sensitivity and excellent specificity for detection of IgA. No significant cross-reactivity or interference between IgA and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IgA and all the analogues, therefore, cross reaction may still exist.

    Sensitivity

    18.75 pg/mL

    Components

    - ELISA Microplate
    - Lyophilized Standard
    - Sample / Standard Dilution Buffer
    - Biotin-labeled Antibody (Concentrated)
    - Antibody Dilution Buffer
    - HRP-Streptavidin Conjugate(SABC)
    - SABC Dilution Buffer
    - TMB Substrate
    - Stop Solution
    - Wash Buffer (25X)
    - Plate Sealer
    - Product Description

    Material not included

    - Microplate reader (wavelength: 450nm)
    - 37 °C incubator
    - Automated plate washer
    - Precision single and multi-channel pipette and disposable tips
    - Clean tubes and Eppendorf tubes
    - Deionized or distilled water
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Sandwich ELISA, Double Antibody

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

    Expiry Date

    6 months
  • Target See all IgA ELISA Kits

    IgA

    Target Type

    Antibody

    Background

    IgA(Immunoglobulin A)
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