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GM-CSF Ab ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GM-CSF Ab.
Catalog No. ABIN5520451

Quick Overview for GM-CSF Ab ELISA Kit (ABIN5520451)

Target

See all GM-CSF Ab products
GM-CSF Ab (Anti-Granulocyte-Macrophage Colony Stimulating Factor Antibody (GM-CSF Ab))

Reactivity

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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.625 ng/mL - 40 ng/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    0.625 ng/mL

    Purpose

    For quantitative detection of GM-CSF Ab in serum, plasma, tissue homogenates.

    Analytical Method

    Quantitative

    Specificity

    This assay has high sensitivity and excellent specificity for detection of GM-CSF Ab. No significant cross- reactivity or interference between GM-CSF Ab and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between GM-CSF Ab and all the analogues, therefore, cross reaction may still exist.

    Sensitivity

    0.375 ng/mL

    Components

    - ELISA Microplate
    - Lyophilized Standard
    - Sample / Standard Dilution Buffer
    - Biotin-labeled Antibody (Concentrated)
    - Antibody Dilution Buffer
    - HRP-Streptavidin Conjugate(SABC)
    - SABC Dilution Buffer
    - TMB Substrate
    - Stop Solution
    - Wash Buffer (25X)
    - Plate Sealer
    - Product Description

    Material not included

    - Microplate reader (wavelength: 450nm)
    - 37 °C incubator
    - Automated plate washer
    - Precision single and multi-channel pipette and disposable tips
    - Clean tubes and Eppendorf tubes
    - Deionized or distilled water
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Sandwich ELISA, Double Antigen

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

    Expiry Date

    6 months
  • Target See all GM-CSF Ab products

    GM-CSF Ab (Anti-Granulocyte-Macrophage Colony Stimulating Factor Antibody (GM-CSF Ab))

    Alternative Name

    GM-CSF Ab

    Target Type

    Antibody, Antibody

    Background

    GM-CSF Ab
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