Endothelin 1 ELISA Kit
Quick Overview for Endothelin 1 ELISA Kit (ABIN5520507)
Target
See all Endothelin 1 (EDN1) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 1.25 pg/mL
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Purpose
- For quantitative detection of EDN1 in serum, plasma, tissue homogenates.
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Analytical Method
- Quantitative
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Specificity
- This assay has high sensitivity and excellent specificity for detection of EDN1. No significant cross-reactivity or interference between EDN1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between EDN1 and all the analogues, therefore, cross reaction may still exist.
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Sensitivity
- 0.75 pg/mL
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Components
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- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description
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Endothelin 1 (EDN1) ELISA Kit
EDN1 Reactivity: Human Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Human Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Mouse Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Rat Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Guinea Pig Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Pig Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Rat Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Saliva, Serum, Urine
Endothelin 1 (EDN1) ELISA KitEDN1 Reactivity: Rabbit Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Sandwich ELISA, Double Antibody
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Sample Volume
- 100 μL
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
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Expiry Date
- 6 months
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- Endothelin 1 (EDN1)
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Alternative Name
- EDN1
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Background
- ET-1/EDN1/ET1/HDLCQ7/PPET1/Preproendothelin-1/endothelin 1/endothelin-1/preproendothelin-1
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UniProt
- P05305
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Pathways
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
Target See all Endothelin 1 (EDN1) ELISA Kits
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