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AAHA ELISA Kit

Human AAHA ELISA Kit Colorimetric assay for quantification of Human AAHA.
Catalog No. ABIN5520621

Quick Overview for AAHA ELISA Kit (ABIN5520621)

Target

See all AAHA products
AAHA (Anti-Apolipoprotein Antibody (AAHA))

Reactivity

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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

3.125 ng/mL - 200 ng/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    3.125 ng/mL

    Purpose

    For quantitative detection of AAHA in serum, plasma, tissue homogenates.

    Analytical Method

    Quantitative

    Specificity

    This assay has high sensitivity and excellent specificity for detection of AAHA. No significant cross-reactivity or interference between AAHA and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between AAHA and all the analogues, therefore, cross reaction may still exist.

    Sensitivity

    1.875 ng/mL

    Components

    - ELISA Microplate
    - Lyophilized Standard
    - Sample / Standard Dilution Buffer
    - Biotin-labeled Antibody (Concentrated)
    - Antibody Dilution Buffer
    - HRP-Streptavidin Conjugate(SABC)
    - SABC Dilution Buffer
    - TMB Substrate
    - Stop Solution
    - Wash Buffer (25X)
    - Plate Sealer
    - Product Description

    Material not included

    - Microplate reader (wavelength: 450nm)
    - 37 °C incubator
    - Automated plate washer
    - Precision single and multi-channel pipette and disposable tips
    - Clean tubes and Eppendorf tubes
    - Deionized or distilled water
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Sandwich ELISA, Double Antigen

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

    Expiry Date

    6 months
  • Target See all AAHA products

    AAHA (Anti-Apolipoprotein Antibody (AAHA))

    Alternative Name

    AAHA

    Background

    AAHA(Anti-Apolipoprotein Antibody)/anti-β2 glycoprotein I antibodies
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