HNE ELISA Kit

Catalog No. ABIN5520635

This Human HNE ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human HNE.

Quick Overview for HNE ELISA Kit (ABIN5520635)

Target: HNE (4-Hydroxynonenal (HNE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details HNE ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: For quantitative detection of 4-HNE in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 4-HNE. No significant cross-reactivity or interference between 4-HNE and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between 4-HNE and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 18.75 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Competitive ELISA, Coated with Antigen

Sample Volume: 50 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for HNE

Target: HNE (4-Hydroxynonenal (HNE))

Alternative Name: 4-HNE

Target Type: Chemical

Background: 4-HNE(4-Hydroxynonenal)/HNE/4 HNE