Sphingosine 1 Phosphate ELISA Kit

Catalog No. ABIN5521213

This Colorimetric ELISA kit is designed for the quantitative measurement of Human . There is 1 publication available.

Quick Overview for Sphingosine 1 Phosphate ELISA Kit (ABIN5521213)

Target: Sphingosine 1 Phosphate

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 3.125 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details

Minimum Detection Limit: 3.125 ng/mL

Purpose: For quantitative detection of Sphingosine 1 Phosphate in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate. No significant cross-reactivity or interference between Sphingosine 1 Phosphate and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Sphingosine 1 Phosphate and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 1.875 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Competition ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References

Aslan, Kıraç, Kaya, Özcan, Salim, Küpesiz: "Decreased Serum Levels of Sphingomyelins and Ceramides in Sickle Cell Disease Patients." in: Lipids, Vol. 53, Issue 3, pp. 313-322, (2018) (PubMed).

Target Details

Target: Sphingosine 1 Phosphate

Background: S1P

UniProt: Q14703