NOS2 ELISA Kit

Catalog No. ABIN5522467

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse NOS2.

Quick Overview for NOS2 ELISA Kit (ABIN5522467)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details NOS2 ELISA Kit

Minimum Detection Limit: 0.313 ng/mL

Purpose: For quantitative detection of NOS2/iNOS in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of NOS2/iNOS. No significant cross- reactivity or interference between NOS2/iNOS and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between NOS2/iNOS and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.188 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: NOS2/iNOS

Background: NOS2/iNOS/NOS2A/HEP-NOS/I-NOS/Hepatocyte NOS/Peptidyl-cysteine S-nitrosylase NOS2/Inducible NO synthase/Inducible NOS/INOS/NOS/NOS, type II/NOS type II

UniProt: P29477

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process